This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-Beta2-PAmcherry1
catalog :
114190
product information
Catalog Number :
114190
Product Name :
pcDNA3.1-Beta2-PAmcherry1
article :
doi
id28196278
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation 2-pamCherry1 was generated by replacing mEos2 in the 2-mEos2 construct by pamCherry1 using the XhoI and ApaI restriction sites. The primers ATCCGC- GAACTCGAGATGGTCAGCAAGGGCGAG (sense) and AGGTCCGAGGGCCCCTTACTTGTACAGCTCGTC (antisense) were used to generate a XhoI and ApaI sites in the pamCherry1 insert by PCR.
backbone_originInvitrogen
backbone_size5424
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
studying clustering of Beta2 receptors by superresolution microscopy (PALM)
origin :
37
pi :
alt_names
Beta2
ADRB2
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5XhoI
promoterSV40
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
geneADRB2
id154
genbank_ids
mutation
nameAdrenergic receptor beta 2
shRNA_sequence
size1326
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
locationN terminal on insert
tag3HA
locationC terminal on insert
tagPAmcherry1
resistance markers :
4077
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA