ORAI1-YFP L273D-L276D | Addgene 114182 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ORAI1-YFP L273D-L276D

catalog :

114182

citations: 1

Reference
Bulla M, Gyimesi G, Kim J, Bhardwaj R, Hediger M, Frieden M, et al. ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain. J Physiol. 2019;597:561-582 pubmed publisher

product information

Catalog Number :

114182

Product Name :

ORAI1-YFP L273D-L276D

article :

doi	10.1113/JP277079
id	28196276
pubmed_id	30382595

bacterial resistance :

Kanamycin

cloning :

backbone

EGFP-N1

backbone_mutation

Generated by site-directed mutagenesis on plasmid Orai1 YFP (Anjana Rao, Addgene #19756).

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CRACM1, IMD9, ORAT1, TAM2, TMEM142A
gene	ORAI1
id	84876

genbank_ids

mutation

L273D/L276D

name

ORAI1-YFP L273D-L276D

shRNA_sequence

size

species

9606

Homo sapiens