This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA5-FRT/TO-FLAG-SHLD3
catalog :
114123
citations: 1
Reference
Noordermeer S, Adam S, Setiaputra D, Barazas M, Pettitt S, Ling A, et al. The shieldin complex mediates 53BP1-dependent DNA repair. Nature. 2018;560:117-121 pubmed publisher
product information
Catalog Number :
114123
Product Name :
pcDNA5-FRT/TO-FLAG-SHLD3
article :
doi10.1038/s41586-018-0340-7
id28196270
pubmed_id30022168
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5-FRT/TO-FLAG
backbone_mutation
backbone_origin
backbone_size5129
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Genomic integration, tetracycline inducible
growth strain :
Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells
origin :
37
pi :
alt_names
CTC543A2.2
RINN1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5AscI
promoterCMV/TetO2
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCTC-534A2.2, RINN1
geneSHLD3
id112441434
genbank_ids
mutation
nameSHLD3
shRNA_sequence
size747
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
2121
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA