This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
KI(GAPDH)-P2A-EGFP-CMV-NeoR
catalog :
114009
citations: 1
Reference
Antonova E, Glazova O, Gaponova A, Eremyan A, Zvereva S, Grebenkina N, et al. Successful CRISPR/Cas9 mediated homologous recombination in a chicken cell line. F1000Res. 2018;7:238 pubmed publisher
product information
Catalog Number :
114009
Product Name :
KI(GAPDH)-P2A-EGFP-CMV-NeoR
article :
doi10.12688/f1000research.13457.2
id28196250
pubmed_id29946437
bacterial resistance :
Ampicillin
cloning :
backboneLSL-Cas9-Rosa26TV
backbone_mutation
backbone_origin
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PmeI
cloning_site_5MauBI
promoter
sequencing_primer_3CTCCTTGGATGCCATGTGGACCATCAAG
sequencing_primer_5TTGTTGACCTGACCTGCCGTCTGGAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKNC-NDS6
geneGAPDH
id374193
genbank_ids
NM_204305.1
mutation
nameGAPDH left arm
shRNA_sequence
size1000
species
9031
Gallus gallus
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5PmeI
promoterendogenous promoter (chicken GAPDH in the case of insertion)
sequencing_primer_3cttgatatcgaattcctgcagggc
sequencing_primer_5ACATGGCATCCAAGGAGggtttaaac
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameeGFP
shRNA_sequence
size700
species
32644
Other
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3ggcgcgcccTAAGATACATTG
sequencing_primer_5ctagtggtgccagggcgtgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameNeoR
shRNA_sequence
size800
species
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SgrDI
cloning_site_5AscI
promoter-
sequencing_primer_3acccggtagaattcgatatcaagc
sequencing_primer_5GCATTCTAGTTGTGGTTTGTCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
NM_204305.1
mutation
nameGAPDH right arm
shRNA_sequence
size3000
species
9031
Gallus gallus
tags
resistance markers :
4071
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA