This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YFP-Rac2(V12)
catalog :
11397
citations: 2
Reference
Fray M, Charpentier J, Sylvain N, Seminario M, Bunnell S. Vav2 lacks Ca2+ entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42. J Cell Sci. 2020;133: pubmed publisher
Hoppe A, Swanson J. Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis. Mol Biol Cell. 2004;15:3509-19 pubmed
product information
Catalog Number :
11397
Product Name :
YFP-Rac2(V12)
article :
doi10.1091/mbc.e03-11-0847
id205
pubmed_id15169870
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-C1
backbone_mutation
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Expresses Rac2(V12) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
origin :
37
pi :
alt_names
Rac2(V12)
YFP
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BglII
promoter
sequencing_primer_3CAGGGGGAGGTGTGGGAGGTTT
sequencing_primer_5CCTGAGCAAAGACCCCAACGAGAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEN-7, Gx, HSPC022, IMD73A, IMD73B, IMD73C, p21-Rac2
geneRAC2
id5880
genbank_ids
AF498965
mutationObtained with Glycine 12 changed to Valine.
nameras-related C3 botulinum toxin substrate 2
shRNA_sequence
size579
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagYFP
plasmid copy :
Human Rac2(G12V) cDNA was obtained from the UMR cDNA Resource Center (www.cdna.org).
resistance markers :
512
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA