This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YFP-Rac1
catalog :
11391
citations: 6
Reference
Bao L, Ren J, Nguyen M, Slusarczyk A, Thole J, Martinez S, et al. The cellular function of ROP GTPase prenylation is important for multicellularity in the moss Physcomitrium patens. Development. 2022;149: pubmed publisher
Wang M, He P, Han Y, Dong L, Yun C. Control of Intestinal Epithelial Permeability by Lysophosphatidic Acid Receptor 5. Cell Mol Gastroenterol Hepatol. 2021;12:1073-1092 pubmed publisher
Fray M, Charpentier J, Sylvain N, Seminario M, Bunnell S. Vav2 lacks Ca2+ entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42. J Cell Sci. 2020;133: pubmed publisher
Singh R, Haka A, Bhardwaj P, Zha X, Maxfield F. Dynamic Actin Reorganization and Vav/Cdc42-Dependent Actin Polymerization Promote Macrophage Aggregated LDL (Low-Density Lipoprotein) Uptake and Catabolism. Arterioscler Thromb Vasc Biol. 2018;:ATVBAHA118312087 pubmed publisher
Baranov M, Revelo N, Dingjan I, Maraspini R, ter Beest M, Honigmann A, et al. SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis. Cell Rep. 2016;17:1518-1531 pubmed publisher
Hoppe A, Swanson J. Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis. Mol Biol Cell. 2004;15:3509-19 pubmed
product information
Catalog Number :
11391
Product Name :
YFP-Rac1
article :
doi10.1091/mbc.e03-11-0847
id205
pubmed_id15169870
bacterial resistance :
Kanamycin
cloning :
backbonepEYFP-C1
backbone_mutation
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Expresses Rac1 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
origin :
37
pi :
alt_names
Rac1
YFP
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BglII
promoter
sequencing_primer_3CAGGGGGAGGTGTGGGAGGTTT
sequencing_primer_5CCTGAGCAAAGACCCCAACGAGAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AF498964
mutation
nameras-related C3 botulinum toxin substrate 1
shRNA_sequence
size580
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagYFP
plasmid copy :
Human Rac1 cDNA was a gift from Klaus Hahn.
resistance markers :
512
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA