pTriEx4-ADCY6-C2 (917-1165 aa) | Addgene 113904 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTriEx4-ADCY6-C2 (917-1165 aa)

catalog :

113904

citations: 1

Reference
Thomas A, Ramananda Y, Mun K, Naren A, Arora K. AC6 is the major adenylate cyclase forming a diarrheagenic protein complex with cystic fibrosis transmembrane conductance regulator in cholera. J Biol Chem. 2018;293:12949-12959 pubmed publisher

product information

Catalog Number :

113904

Product Name :

pTriEx4-ADCY6-C2 (917-1165 aa)

article :

doi	10.1074/jbc.RA118.003378
id	28196230
pubmed_id	29903911

bacterial resistance :

Ampicillin

cloning :

backbone

pTriEx-4

backbone_mutation

None

backbone_origin

Novagen

backbone_size

5238

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

Insect Expression

growth notes :

Please acknowledge the Principal Investigator, cite the article in which the plasmids were described. Addgene NGS found a V1002A amino acid change within the ADCY6 translation. The depositing laboratory affirms that V1002A does not affect the binding capability of the protein to CFTR that was published in their JBC paper, though they have not tested the function of this construct

growth strain :

His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7, CMV
sequencing_primer_3	TriEx Dn
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AC6, LCCS8
gene	ADCY6
id	112

genbank_ids

mutation

Please see Depositor Comments

name

Adenylate Cyclase 6 cytoplasmic catalytic domain 2

shRNA_sequence

size

747

species

9606

Homo sapiens