This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p3E_he1a:mCherry
catalog :
113881
product information
Catalog Number :
113881
Product Name :
p3E_he1a:mCherry
article :
doi
id28196227
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepDONR P2R-P3
backbone_mutation
backbone_originInvitrogen
backbone_size2640
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Danio expression
growth notes :
Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:mRFP-NTRmut;HE-mCherry Reference for the he1a promoter was first described in: Xie et al., Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression in a NRSF/REST-dependent manner . BMC Biology 2012 10:93 https://doi.org/10.1186/1741-7007-10-93
growth strain :
p3E entry vector for Three Multisite Gateway
growth temp :
10 ug/ml kanamycin final concentration of LB media
origin :
37
pi :
alt_names
hatching gland promoter mCherry
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasescb284, he1, he1a, wu:cegs2099, zgc:111847
genehe1.1
id569018
genbank_ids
mutation
namehe1a
shRNA_sequence
size1749
species
7955
Danio rerio
tags
locationC terminal on insert
tagmCherry
resistance markers :
2163
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA