This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pARF1(Q71L)-CFP
catalog :
11385
citations: 2
Reference
Rafiq N, Lieu Z, Jiang T, Yu C, Matsudaira P, Jones G, et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol. 2017;216:181-197 pubmed publisher
Beemiller P, Hoppe A, Swanson J. A phosphatidylinositol-3-kinase-dependent signal transition regulates ARF1 and ARF6 during Fcgamma receptor-mediated phagocytosis. PLoS Biol. 2006;4:e162 pubmed
product information
Catalog Number :
11385
Product Name :
pARF1(Q71L)-CFP
article :
doi10.1371/journal.pbio.0040162
id686
pubmed_id16669702
bacterial resistance :
Kanamycin
cloning :
backbonepECFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Encodes a CFP fusion to the C-terminus of constitutively-active ARF1. CFP is the monomeric (A207K) version of ECFP.
origin :
37
pi :
alt_names
ARF1(Q71L)
CFP
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoter
sequencing_primer_3CCCCGGTGAACAGCTCCTCGCCCTTGCTCA
sequencing_primer_5GGTGGGAGGTCTATATAAGCAGAGCTGGTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPVNH8
geneARF1
id375
genbank_ids
AF493881
mutationChanged Glutamine 71 to Leucine.
nameADP Ribosylation Factor 1
shRNA_sequence
size545
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagCFP
plasmid copy :
hARF1 was obtained from the UMR cDNA Resource center (www.cDNA.org) and mutagenized using site-directed mutagenesis.
resistance markers :
512
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA