This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pARF1-CFP
catalog :
11381
citations: 4
Reference
Swart T, Khan F, Ntlantsana A, Laming D, Veale C, Przyborski J, et al. Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay. Sci Rep. 2020;10:4193 pubmed publisher
Rafiq N, Lieu Z, Jiang T, Yu C, Matsudaira P, Jones G, et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol. 2017;216:181-197 pubmed publisher
Stafa K, Trancikova A, Webber P, Glauser L, West A, Moore D. GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1. PLoS Genet. 2012;8:e1002526 pubmed publisher
Beemiller P, Hoppe A, Swanson J. A phosphatidylinositol-3-kinase-dependent signal transition regulates ARF1 and ARF6 during Fcgamma receptor-mediated phagocytosis. PLoS Biol. 2006;4:e162 pubmed
product information
Catalog Number :
11381
Product Name :
pARF1-CFP
article :
doi10.1371/journal.pbio.0040162
id686
pubmed_id16669702
bacterial resistance :
Kanamycin
cloning :
backbonepECFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Encodes a CFP fusion to the C-terminus of ARF1. CFP is the monomeric (A207K) version of ECFP.
origin :
37
pi :
alt_names
ARF1
CFP
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoter
sequencing_primer_3CCCCGGTGAACAGCTCCTCGCCCTTGCTCA
sequencing_primer_5GGTGGGAGGTCTATATAAGCAGAGCTGGTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPVNH8
geneARF1
id375
genbank_ids
AF493881
mutation
nameADP Ribosylation Factor 1
shRNA_sequence
size545
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagCFP
plasmid copy :
hARF1 was obtained from the UMR cDNA Resource center (www.cDNA.org).
resistance markers :
512
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA