This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pR BirA
catalog :
113640
citations: 1
Reference
Moon J, Pepper M. Generation of Allergen-Specific Tetramers for a Murine Model of Airway Inflammation. Methods Mol Biol. 2018;1799:165-181 pubmed publisher
product information
Catalog Number :
113640
Product Name :
pR BirA
article :
doi10.1007/978-1-4939-7896-0_14
id28196189
pubmed_id29956152
bacterial resistance :
Ampicillin
cloning :
backbonepRMHa-3
backbone_mutationpoint mutation at 1796 to ablate second SpeI restriction site
backbone_origin
backbone_size3830
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
This gene encodes a biotin ligase that mediates in vivo biotinylation of BirA signal sequences on recombinant proteins co-expressed in the same cells.
growth strain :
Insect cell expression vector for BirA Biotin Ligase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterMetallothionein
sequencing_primer_3CTGCCGCTCCCATTTATCTAT
sequencing_primer_5TGTGCAAAAGAGGTGAATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb3973, ECK3965, bioR, dhbB
genebirA
id948469
genbank_ids
mutation
nameBirA Biotin Ligase
shRNA_sequence
size972
species
tags
plasmid copy :
William Kwok, Benaroya Research Institute, Seattle, WA.
resistance markers :
4053
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA