pBABE puro mouse IRS-4 | Addgene 11363 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABE puro mouse IRS-4

catalog :

11363

citations: 2

Reference
Dörpholz G, Murgai A, Jatzlau J, Horbelt D, Belverdi M, Heroven C, et al. IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation. Sci Rep. 2017;7:8778 pubmed publisher
Tsuruzoe K, Emkey R, Kriauciunas K, Ueki K, Kahn C. Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Mol Cell Biol. 2001;21:26-38 pubmed

product information

Catalog Number :

11363

Product Name :

pBABE puro mouse IRS-4

article :

doi	10.1128/MCB.21.1.26-38.2001
id	526
pubmed_id	11113178

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE puro

backbone_mutation

backbone_origin

pBABE-puro is available at Addgene

backbone_size

5169

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Mouse IRS-4 genomic DNA was screened from the BAC genomic DNA library (Genome Systems Inc. St. Louis, Mo.) by PCR with specific primers for mouse IRS-4 genomic DNA. The AvrII-ClaI fragment encompassing the entire exon 1 of mouse IRS-4 gene was blunt ended and ligated into the SnaBI site in pBABE-puro.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SnaBI
cloning_site_5	SnaBI
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	IRS-4
gene	Irs4
id	16370

genbank_ids

NM_010572

mutation

name

IRS-4

shRNA_sequence

size

3700

species

10090

Mus musculus