This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLexA::Ras
catalog :
11340
citations: 1
Reference
Chang E, Barr M, Wang Y, Jung V, Xu H, Wigler M. Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell. 1994;79:131-41 pubmed
product information
Catalog Number :
11340
Product Name :
pLexA::Ras
article :
doi10.1016/0092-8674(94)90406-5
id523
pubmed_id7923372
bacterial resistance :
Ampicillin
cloning :
backbonepLexA
backbone_mutation
backbone_originsee pLexA map in author's map
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
cDNA of fission yeast S. pombe Ras1 gene (oncogene) cloned into the yeast two-hybrid DNA-binding domain vector pLexA (ref., Chang et al., 2004, Cell, 79:131-141). See author's map for picture of empty pLexA vector.
origin :
37
pi :
alt_names
Ras-1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPAC17H9.09c, ste5
generas1
id2542285
genbank_ids
mutation
nameRas1
shRNA_sequence
size660
species
4896
Schizosaccharomyces pombe
tags
locationN terminal on backbone
tagLexA DNA binding domain
resistance markers :
582
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA