This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLexA::nud-1
catalog :
11339
product information
Catalog Number :
11339
Product Name :
pLexA::nud-1
article :
doi
id510
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepLexA
backbone_mutation
backbone_originsee pLexA map in author's map
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See author's map for picture of empty pLexA vector. Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F53A2.4
genenud-1
id176754
genbank_ids
NM_067348
mutation
namenud-1
shRNA_sequence
size963
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tagLexA DNA Binding Domain
resistance markers :
59
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA