This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
lis-1::L4440
catalog :
11336
product information
Catalog Number :
11336
Product Name :
lis-1::L4440
article :
doi
id510
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneL4440
backbone_mutation
backbone_originL4440 is available at Addgene
backbone_size2790
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
RNAi
growth notes :
Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5L4440 primer (AGCGAGTCAGTGAGCGAG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genelis-1
id176758
genbank_ids
NM_067354
mutation
namelis-1
shRNA_sequence
size1200
species
6239
Caenorhabditis elegans
tags
plasmid copy :
Dr. Andrew Fire created the vector backbone L4440
resistance markers :
59
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA