This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PAK4-FL-3'UTR-MT2
catalog :
113093
citations: 1
Reference
Phatak P, Burrows W, Chesnick I, Tulapurkar M, Rao J, Turner D, et al. MiR-199a-3p decreases esophageal cancer cell proliferation by targeting p21 activated kinase 4. Oncotarget. 2018;9:28391-28407 pubmed publisher
product information
Catalog Number :
113093
Product Name :
PAK4-FL-3'UTR-MT2
article :
doi10.18632/oncotarget.25375
id28196091
pubmed_id29983868
bacterial resistance :
Ampicillin
cloning :
backbonepmirGLO Dual-Luciferase
backbone_mutation
backbone_originPromega
backbone_size7350
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Reverse: GAGGAGGCTGTCCAGGTGTGTCAGTCATTTGCTGCAGGGG
CCCCTGCAGCAAATGACTGACACACCTGGACAGCCTCCT
C
Oligos used for site-directed mutagenesis Forward:
growth strain :
Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated
origin :
37
pi :
alt_names
PAK4
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5DraI
promoterPGK
sequencing_primer_3GTCCAAACTCATCAATGTA
sequencing_primer_5ACACGGTAAAACCATGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genePAK4
id10298
genbank_ids
NM_001014834.2
NM_001014834.2
mutationSecond predicted miR-199a-3p binding site mutated by substitution of 4 bases
namep21 Activated kinase 4
shRNA_sequence
size507
species
9606
Homo sapiens
tags
resistance markers :
4033
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA