pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9) | Addgene 113039 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

catalog :

113039

citations: 3

Reference
Aschenbrenner S, Kallenberger S, Hoffmann M, Huck A, Eils R, Niopek D. Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity. Sci Adv. 2020;6:eaay0187 pubmed publisher
Hoffmann M, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, et al. Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Nucleic Acids Res. 2019;: pubmed publisher
Bubeck F, Hoffmann M, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer M, et al. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018;15:924-927 pubmed publisher

product information

Catalog Number :

113039

Product Name :

pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

article :

doi	10.1038/s41592-018-0178-9
id	28196068
pubmed_id	30377362

bacterial resistance :

Ampicillin

cloning :

backbone

scAAV vector

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth notes :

Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362

growth strain :

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

origin :

pi :

alt_names
cloning
entrez_gene
genbank_ids
mutation
name	2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
shRNA_sequence
size
species
tags

resistance markers :

4114

tags :

High Copy