This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
rd
catalog :
112912
citations: 1
Reference
Chen L, Brown J, Mok Y, Hatters D, McKnight C. The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). J Biol Chem. 2013;288:8313-20 pubmed publisher
product information
Catalog Number :
112912
Product Name :
rd
article :
doi10.1074/jbc.M112.438861
id28196055
pubmed_id23355471
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2T
backbone_mutation
backbone_originGE Healthcare
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag
origin :
37
pi :
, and 5 -CCGGCTGTCCGCCCACACCTTCCCGGCTCCGCCCCCCGCTGCGGCTTTGGGTGACAGCGAGCGC-3 were used to replace 89KSTSPPPSPEVWAD102 with 89KAAAGGGAGKVWAD102 namedematinshRNA_sequencesize1150species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGlutatione-S-Tranferase
location
tagprecision protease site added between GST and dematin

-GCGCTCGCTGTCACCCAAAGCCGCAGCGGGGGGCGGAG
CCGGGAAGGTGTGGGCGGACAGCCGG-3
alt_names
protein 4.9
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterT7
sequencing_primer_3pGEX_3_primer
sequencing_primer_5M13_pUC_rev_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDMT, EPB49
geneDMTN
id2039
genbank_ids
NM_001252664
mutationPEST sequence near the N-terminal removed. 5
plasmid copy :
Athar Chishti, Tufts University
resistance markers :
4020
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA