This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmTHC_PGKneoLox2DTA.2
catalog :
112624
citations: 1
Reference
Hartman B, BÓ§scke R, Ellwanger D, Keymeulen S, Scheibinger M, Heller S. Fbxo2VHC mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol. 2018;443:64-77 pubmed publisher
product information
Catalog Number :
112624
Product Name :
pmTHC_PGKneoLox2DTA.2
article :
doi10.1016/j.ydbio.2018.08.013
id28195969
pubmed_id30179592
bacterial resistance :
Ampicillin
cloning :
backbonePGKneoLox2DTA.2
backbone_mutation
backbone_originP. Soriano Addgene Plasmid 13449
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
Cre/Lox
growth strain :
H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
origin :
37
pi :
alt_names
HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
mTFP1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3CCGCTTCCCTTGTACAGCTCGT
sequencing_primer_5TTTATTCATGAGGCCGGCCACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021058
mutation
nameH2B-mTFP1
shRNA_sequence
size1101
species
9606
Homo sapiens
32644
Other
Anthozoa
tags
locationC terminal on insert
tagmTeal (mTFP1)
alt_names
HygroR
hygromycin-B
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3ttcctttgccctcggacga
sequencing_primer_5atgaaaaagcctgaactcaccgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
XM_003071606
mutation
nameHygrophosphotransferase
shRNA_sequence
size1023
species
32644
Other
Coccidioides posadasii C735 delta SOWgp
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3TCAAGCTGTGGCAGGGAAACC
sequencing_primer_5ATGTCCAATTTACTGACCGTACA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre-ERT2 fusion protein
shRNA_sequence
size1983
species
32644
Other
Bacteriophage P1
tags
plasmid copy :
H2B was cloned from Addgene plasmid 26750, mTeal was cloned from Addgene plasmid 54553, HygroR was cloned from Addgene plasmid 28226, CreERT2 and Rabbit betaglobin PolyA were cloned from Addgene plasmid 14797, PGKneoLox2DTA.2 backbone was cloned from Addgene plasmid 13449
resistance markers :
4072
tags :
High Copy
terms :
Neomycin (select with G418)
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA