This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-EHC
catalog :
112619
citations: 1
Reference
Hartman B, BÓ§scke R, Ellwanger D, Keymeulen S, Scheibinger M, Heller S. Fbxo2VHC mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol. 2018;443:64-77 pubmed publisher
product information
Catalog Number :
112619
Product Name :
pCAG-EHC
article :
doi10.1016/j.ydbio.2018.08.013
id28195969
pubmed_id30179592
bacterial resistance :
Kanamycin
cloning :
backbonepromoterless backbone from pd2EGFP-1
backbone_mutationCAG promoter inserted at MCS
backbone_originClontech
backbone_size3110
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
growth notes :
CAG promoter driving multicistronic reporter plasmid containing H2BEmerald, HygroR, and CreERT2 in a single ORF with two P2A sequences.
growth strain :
expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
origin :
37
pi :
alt_names
HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3TGAAGTTAGTAGCTCCGCTTCC
sequencing_primer_5AAAGAATTCATGAGGCCGGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021058
mutation
nameH2B-Emerald
shRNA_sequence
size1101
species
9606
Homo sapiens
32644
Other
Aequorea victoria
tags
locationC terminal on insert
tagVenus
alt_names
HygroR
hygromycin-B
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3ttcctttgccctcggacga
sequencing_primer_5atgaaaaagcctgaactcaccgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
XM_003071606
mutation
nameHygrophosphotransferase
shRNA_sequence
size1023
species
32644
Other
Coccidioides posadasii C735 delta SOWgp
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3TCAAGCTGTGGCAGGGAAACC
sequencing_primer_5ATGTCCAATTTACTGACCGTACA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre-ERT2 fusion protein
shRNA_sequence
size1983
species
32644
Other
Bacteriophage P1
tags
plasmid copy :
H2B was cloned from Addgene plasmid 26750, Emerald was cloned from Addgene plasmid HygroR was cloned from Addgene plasmid 5397628226, CreERT2 and Rabbit betaglobin PolyA were cloned from Addgene plasmid 14797, the CAG promoter was cloned from Addgene plasmid 14757
resistance markers :
4072
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA