This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pVHC
catalog :
112614
citations: 1
Reference
Hartman B, BÓ§scke R, Ellwanger D, Keymeulen S, Scheibinger M, Heller S. Fbxo2VHC mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol. 2018;443:64-77 pubmed publisher
product information
Catalog Number :
112614
Product Name :
pVHC
article :
doi10.1016/j.ydbio.2018.08.013
id28195969
pubmed_id30179592
bacterial resistance :
Kanamycin
cloning :
backbonepromoterless backbone from pd2EGFP-1
backbone_mutation
backbone_originClontech
backbone_size3110
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Promoterless vector, ready for promoter for mammalian expression
growth notes :
Promoterless multicistronic reporter plasmid containing H2BVenus, HygroR, and CreERT2 in a single ORF with two P2A sequences.
growth strain :
promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
origin :
37
pi :
alt_names
HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3TGAAGTTAGTAGCTCCGCTTCC
sequencing_primer_5TTTATTCATGAGGCCGGCCACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021058
mutation
nameH2B-Venus
shRNA_sequence
size1101
species
9606
Homo sapiens
32644
Other
Aequorea victoria
tags
locationC terminal on insert
tagVenus
alt_names
HygroR
hygromycin-B
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3ttcctttgccctcggacga
sequencing_primer_5atgaaaaagcctgaactcaccgc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
XM_003071606
mutation
nameHygrophosphotransferase
shRNA_sequence
size1023
species
32644
Other
Coccidioides posadasii C735 delta SOWgp
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNo Promoter
sequencing_primer_3TCAAGCTGTGGCAGGGAAACC
sequencing_primer_5ATGTCCAATTTACTGACCGTACA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre-ERT2 fusion protein
shRNA_sequence
size1983
species
32644
Other
Bacteriophage P1
tags
plasmid copy :
H2B-Venus was cloned from Addgene plasmid 26750, HygroR was cloned from Addgene plasmid 28226, CreERT2 and Rabbit betaglobin PolyA were cloned from Addgene plasmid 14797
resistance markers :
4072
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA