This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-hygro CDK4 R24C
catalog :
11254
citations: 4
Reference
Tran E, Shi T, Li X, Chowdhury A, Jiang D, Liu Y, et al. Development of human alveolar epithelial cell models to study distal lung biology and disease. iScience. 2022;25:103780 pubmed publisher
Jang J, Han D, Golkaram M, Audouard M, Liu G, Bridges D, et al. Control over single-cell distribution of G1 lengths by WNT governs pluripotency. PLoS Biol. 2019;17:e3000453 pubmed publisher
Schmit F, Utermark T, Zhang S, Wang Q, Von T, Roberts T, et al. PI3K isoform dependence of PTEN-deficient tumors can be altered by the genetic context. Proc Natl Acad Sci U S A. 2014;111:6395-400 pubmed publisher
Hahn W, Dessain S, Brooks M, King J, Elenbaas B, Sabatini D, et al. Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Mol Cell Biol. 2002;22:2111-23 pubmed
product information
Catalog Number :
11254
Product Name :
pBABE-hygro CDK4 R24C
article :
doi10.1128/MCB.22.7.2111-2123.2002
id199
pubmed_id11884599
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-hygro
backbone_mutation
backbone_origin
backbone_size5558
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
R24C allele of the CDK4 gene encodes a kinase that is insensitive to inhibition by p16INK4A and p15INK4B, the major negative regulators of CDK4.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMM3, PSK-J3
geneCDK4
id1019
genbank_ids
mutationR24C
nameCDK4
shRNA_sequence
size1000
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA