Adeno-miR-27b | Addgene 112539 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Adeno-miR-27b

catalog :

112539

citations: 2

Reference
Gorbea C, Elhakiem A, Cazalla D. Allosteric regulation of noncoding RNA function by microRNAs. Nucleic Acids Res. 2022;50:6511-6520 pubmed publisher
Zhou Q, Gallagher R, Ufret Vincenty R, Li X, Olson E, Wang S. Regulation of angiogenesis and choroidal neovascularization by members of microRNA-23~27~24 clusters. Proc Natl Acad Sci U S A. 2011;108:8287-92 pubmed publisher

product information

Catalog Number :

112539

Product Name :

Adeno-miR-27b

article :

doi	10.1073/pnas.1105254108
id	28195959
pubmed_id	21536891

bacterial resistance :

Kanamycin

cloning :

backbone

pAdEasy

backbone_mutation

backbone_origin

Agilent

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Adenoviral

growth notes :

clone into pShuttle-CMV using KpnI/NotI first. There exists a deletion of AGC at positions 285-287 compared to the depositor sequence that the depositing lab confirms is not of concern for function.

growth strain :

Adenovirus overexpression

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIR-27b, MIRN27B, miRNA27B
gene	MIR27B
id	407019

genbank_ids

mutation

name

miR-27b

shRNA_sequence

size

291

species

9606

Homo sapiens