pIGAhRC | Addgene 112510 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pIGAhRC

catalog :

112510

citations: 1

Reference
Miller C. A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures. Toxicol Appl Pharmacol. 1999;160:297-303 pubmed

product information

Catalog Number :

112510

Product Name :

pIGAhRC

article :

doi	10.1006/taap.1999.8769
id	28195987
pubmed_id	10544064

bacterial resistance :

Ampicillin

cloning :

backbone

unknown

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Synthetic Biology

growth notes :

This plasmid contains the human aryl hydrocarbon receptor (AhR) expressed from the GAL1,10 promoter along with ARNT. Together they form a heterodimeric transcription factor. Use the HpaI site for linearization and integration into yeast chromosome III. This is an integrating vector without a yeast replication origin. Addgene NGS detected a K298R mutation within the HIF1B translation. The depositing laboratory states that this does not adversely affect protein function

growth strain :

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	Gal1,10
sequencing_primer_3
sequencing_primer_5	GAL1
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2
gene	ARNT
id	405

aliases	RP85, bHLHe76
gene	AHR
id	196

genbank_ids

mutation

name

HIF1B, AhR

shRNA_sequence

size

species

9606

Homo sapiens

4932

Saccharomyces cerevisiae