This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCUX1.1.0-gDNA
catalog :
112434
citations: 1
Reference
Cao Y, Deng B, Zhang S, Gao H, Song P, Zhang J, et al. Astragalus polysaccharide regulates brown adipogenic differentiation through miR-1258-5p-modulated cut-like homeobox 1 expression. Acta Biochim Biophys Sin (Shanghai). 2021;53:1713-1722 pubmed publisher
product information
Catalog Number :
112434
Product Name :
pCUX1.1.0-gDNA
article :
doi
id28195944
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepX330
backbone_mutation
backbone_originZhang lab (Addgene plasmid ID: 42230)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pCUX1-donor.
growth strain :
CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, Nbla10317, p100, p110, p200, p75
geneCUX1
id1523
genbank_ids
mutation
nameCUX1
shRNA_sequenceCCTCAGAACTCCCATTCGAT AGG
size
species
9606
Homo sapiens
tags
resistance markers :
3990
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA