gfp-Arkadia/RNF111 | Addgene 112228 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

gfp-Arkadia/RNF111

catalog :

112228

citations: 1

Reference
Mavrakis K, Andrew R, Lee K, Petropoulou C, Dixon J, Navaratnam N, et al. Arkadia enhances Nodal/TGF-beta signaling by coupling phospho-Smad2/3 activity and turnover. PLoS Biol. 2007;5:e67 pubmed

product information

Catalog Number :

112228

Product Name :

gfp-Arkadia/RNF111

article :

doi	10.1371/journal.pbio.0050067
id	28195907
pubmed_id	17341133

bacterial resistance :

Ampicillin

cloning :

backbone

pTriEx2-hygro

backbone_mutation

backbone_origin

Novagen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The GFP tagged Arkadia constructs were generated by eliminating the first 9 aa of Arkadia and fusing in frame to GFP Sequences and cloned into SmaI/XhoI of the pTriEx2-hygro (Novagen, http://www.novagen.com) vector.

growth strain :

Expresses GFP-tagged Arkadia in mammalian cells

origin :

pi :

alt_names

Arkadia

Rnf111

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	SmaI
promoter
sequencing_primer_3
sequencing_primer_5	pCAG-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARK, Arkadia
gene	Rnf111
id	93836

genbank_ids

mutation

name

(Arkadia)Rnf111

shRNA_sequence

size

species

10090

Mus musculus