LUC2 (L/Sec/deltaP) | Addgene 112145 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LUC2 (L/Sec/deltaP)

catalog :

112145

citations: 1

Reference
Mehta A, Rebsch C, Kinzy S, Fletcher J, Copeland P. Efficiency of mammalian selenocysteine incorporation. J Biol Chem. 2004;279:37852-9 pubmed

product information

Catalog Number :

112145

Product Name :

LUC2 (L/Sec/deltaP)

article :

doi	10.1074/jbc.M404639200
id	28195873
pubmed_id	15229221

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

origin :

pi :

alt_names

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Gshpx-4, Phgpx, gpx-4, snGpx
gene	Gpx4
id	29328

genbank_ids

mutation

Cysteine 258 mutated to selenocysteine (U) and deleted AUGA PHGPx SECIS

name

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core

shRNA_sequence

size

species

10116

Rattus norvegicus

32644

Other

Photinus pyralis