LUC01 (L/Sec/P) | Addgene 112144 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LUC01 (L/Sec/P)

catalog :

112144

citations: 2

Reference
Chung C, Miller C, Soll D, Krahn N. Introducing Selenocysteine into Recombinant Proteins in Escherichia coli. Curr Protoc. 2021;1:e54 pubmed publisher
Mehta A, Rebsch C, Kinzy S, Fletcher J, Copeland P. Efficiency of mammalian selenocysteine incorporation. J Biol Chem. 2004;279:37852-9 pubmed

product information

Catalog Number :

112144

Product Name :

LUC01 (L/Sec/P)

article :

doi	10.1074/jbc.M404639200
id	28195873
pubmed_id	15229221

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

origin :

pi :

alt_names

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Gshpx-4, Phgpx, gpx-4, snGpx
gene	Gpx4
id	29328

genbank_ids

mutation

Cysteine 258 mutated to selenocysteine (U)

name

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR

shRNA_sequence

size

species

10116

Rattus norvegicus

32644

Other

Photinus pyralis