This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLL3 sgRNA(MS2)-MS2-AID3f
catalog :
112129
citations: 1
Reference
Liu L, Huang M, Dai P, Liu T, Fan S, Cheng X, et al. Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018;25:884-892.e3 pubmed publisher
product information
Catalog Number :
112129
Product Name :
pLL3 sgRNA(MS2)-MS2-AID3f
article :
doi10.1016/j.celrep.2018.09.090
id28195855
pubmed_id30355495
bacterial resistance :
Ampicillin
cloning :
backbonepX330
backbone_mutation
backbone_originZhang lab (Addgene plasmid #42230)
backbone_size7115
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
Additional reference: https://www.ncbi.nlm.nih.gov/pubmed/19543289
growth strain :
empty sgRNA cloning vector with MS2-AID3f
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterhU6,CBh
sequencing_primer_3GGGAGGGGCAAACAACAGAT
sequencing_primer_5CATCGCCGCTAACTCAGGTAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAID, ARP2, CDA2, HEL-S-284, HIGM2
geneAICDA
id57379
genbank_ids
mutationN- mutated and C- terminal truncated, F115Y/C116F/E117W/R119T/deletion of K120/A121D/E122Y/P123Q/L181Q mutant in human AID
nameAIDmono 3f mutant
shRNA_sequence
size531
species
9606
Homo sapiens
tags
resistance markers :
3974
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA