pMal-Abl-PRM 5R | Addgene 112088 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMal-Abl-PRM 5R

catalog :

112088

citations: 2

Reference
Mondal S, Narayan K, Botterbusch S, Powers I, Zheng J, James H, et al. Multivalent interactions between molecular components involved in fast endophilin mediated endocytosis drive protein phase separation. Nat Commun. 2022;13:5017 pubmed publisher
Li P, Banjade S, Cheng H, Kim S, Chen B, Guo L, et al. Phase transitions in the assembly of multivalent signalling proteins. Nature. 2012;483:336-40 pubmed publisher

product information

Catalog Number :

112088

Product Name :

pMal-Abl-PRM 5R

article :

doi	10.1038/nature10879
id	28195843
pubmed_id	22398450

bacterial resistance :

Ampicillin

cloning :

backbone

pMal-tev

backbone_mutation

tev site added on either side of NdeI / BamHI cloning sites, His-6 added to C-term after tev site

backbone_origin

NEB

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

origin :

pi :

alt_names

5 repeats of the protein rich motif from proto-oncogene tyrosine-protein kinase (ABL)

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NdeI
promoter	Lac
sequencing_primer_3	pMAL-Rev: 5'-GTAAAACGACGGCCAGT-3'
sequencing_primer_5	pMAL-Fwd: 5'-TGCGTACTGCGGTGATCAAC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
gene	ABL1
id	25

genbank_ids

AAB60394

mutation

construct contains only the PRM motif of Abl1

name

PRM-5R

shRNA_sequence

size

435

species

9606

Homo sapiens