This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMal_Abl-PRM 3R
catalog :
112086
citations: 1
Reference
Li P, Banjade S, Cheng H, Kim S, Chen B, Guo L, et al. Phase transitions in the assembly of multivalent signalling proteins. Nature. 2012;483:336-40 pubmed publisher
product information
Catalog Number :
112086
Product Name :
pMal_Abl-PRM 3R
article :
doi10.1038/nature10879
id28195843
pubmed_id22398450
bacterial resistance :
Ampicillin
cloning :
backbonepMal-tev
backbone_mutationtev sites added on both sides of MCS, His-6 after c-terminal tev site
backbone_originNEB
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
origin :
37
pi :
alt_names
3 repeats of the protein rich motif from proto-oncogene tyrosine-protein kinase (ABL)
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterLac
sequencing_primer_3pMAL-Rev: 5'-CTGCAAGGCGATTAAGTTGG-3'
sequencing_primer_5pMAL-Fwd: 5'-TGCGTACTGCGGTGATCAAC-3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
geneABL1
id25
genbank_ids
AH005332.2
AAB60394
mutationconstruct contains only the PRM motif of Abl1
nameAbl-PRM-3R
shRNA_sequence
size285
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMBP
locationC terminal on backbone
tagHis-6
resistance markers :
4315
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA