This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKC-4.06
catalog :
112084
citations: 4
Reference
Soederberg A, Mei xdf geier T, Bosserhoff A, Linck Paulus L. MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases Nonsense-Mediated Decay Activity and Promotes Apoptosis via Upregulation of GADD45A. Cells. 2022;11: pubmed publisher
Udy D, Bradley R. Nonsense-mediated mRNA decay uses complementary mechanisms to suppress mRNA and protein accumulation. Life Sci Alliance. 2022;5: pubmed publisher
Kim Guisbert K, Mossiah I, Guisbert E. Titration of SF3B1 Activity Reveals Distinct Effects on the Transcriptome and Cell Physiology. Int J Mol Sci. 2020;21: pubmed publisher
Baird T, Cheng K, Chen Y, Buehler E, Martin S, Inglese J, et al. ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. elife. 2018;7: pubmed publisher
product information
Catalog Number :
112084
Product Name :
pKC-4.06
article :
doi33178 [pii]
id28195842
pubmed_id29528287
bacterial resistance :
Ampicillin
cloning :
backbonep3xFLAG-CMV-10
backbone_mutation
backbone_origin
backbone_size6204
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)
origin :
37
pi :
alt_names
FLuc NMD sensor
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3hgH-PA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD113t-C, ECYT6, beta-globin
geneHBB
id3043
genbank_ids
mutation
name3XFLAG firefly luciferase-beta-globin 39PTC
shRNA_sequence
size2513
species
9606
Homo sapiens
10090
Mus musculus
32644
Other
Photinus pyralis
tags
locationN terminal on insert
tagFLAG
plasmid copy :
b-globin sequence used in this clone was obtained from Professor Lynne Maquat
resistance markers :
1387
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA