This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AT-U1
catalog :
112059
citations: 2
Reference
Jiang L, Xie X, Su N, Zhang D, Chen X, Xu X, et al. Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells. Nat Methods. 2023;20:1563-1572 pubmed publisher
Braselmann E, Wierzba A, Polaski J, Chromiński M, Holmes Z, Hung S, et al. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells. Nat Chem Biol. 2018;14:964-971 pubmed publisher
product information
Catalog Number :
112059
Product Name :
AT-U1
article :
doi10.1038/s41589-018-0103-7
id28195834
pubmed_id30061719
bacterial resistance :
Ampicillin
cloning :
backboneU1 human
backbone_mutation
backbone_origin Reduction of Target Gene Expression by a Modified U1 snRNA (Beckley et al, 2001, Mol Cell Bio)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
In partial sequence, 1 copy of Riboglow (truncated version A)--U1 sequence is in lower case letters, Riboglow sequence is in upper case letters
growth strain :
U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)
origin :
37
pi :
alt_names
NCBI Reference Sequence: NR_004430.2
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5BglII
promoter
sequencing_primer_3U1-H-Seq_RV (5'-GGAAAGCGCGAACGCAGTC-3')
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSD1, HU1-1, RNU1, RNU1A, RNU1A3, RNU1G4, Rnu1a1, U1, U1A1
geneRNU1-1
id26871
genbank_ids
mutation1 copy of Riboglow tag (truncated variant A) near 5' end of U1
nameU1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
1227
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA