This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ACTB-(A)4x
catalog :
112058
citations: 2
Reference
Sarfraz N, Moscoso E, Oertel T, Lee H, Ranjit S, Braselmann E. Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM). Nat Commun. 2023;14:867 pubmed publisher
Braselmann E, Wierzba A, Polaski J, Chromiński M, Holmes Z, Hung S, et al. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells. Nat Chem Biol. 2018;14:964-971 pubmed publisher
product information
Catalog Number :
112058
Product Name :
ACTB-(A)4x
article :
doi10.1038/s41589-018-0103-7
id28195834
pubmed_id30061719
bacterial resistance :
Kanamycin
cloning :
backboneC1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
4 copies of Riboglow (variant A), separated by restriction sites as follows: KpnI - A - KpnI - A - BamHI - A - BamHI - A - BamHI
growth strain :
ACTB mRNA tagged with 4 copies of Riboglow (variant A)
origin :
37
pi :
alt_names
NCBI Reference Sequence: NM_001101.3
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3Quintara Bio: SV50pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5Quintara Bio: CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutation4 copies of Riboglow tag after stop codon
nameACTB
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tag Riboglow RNA tag
resistance markers :
1227
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA