This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RAB11A-GFP HDRT Source (pTR 143)
catalog :
112012
citations: 6
Reference
Chang J, Wang C, Lin S. Interrogation of human microglial phagocytosis by CRISPR genome editing. Front Immunol. 2023;14:1169725 pubmed publisher
M xf6 ller L, Aird E, Schr xf6 der M, Kobel L, Kissling L, van de Venn L, et al. Recursive Editing improves homology-directed repair through retargeting of undesired outcomes. Nat Commun. 2022;13:4550 pubmed publisher
Hoffman H, Aguilar R, Clark A, Groves N, Pezeshkian N, Bruns M, et al. Endocytosed HIV-1 Envelope Glycoprotein Traffics to Rab14+ Late Endosomes and Lysosomes to Regulate Surface Levels in T-Cell Lines. J Virol. 2022;:e0076722 pubmed publisher
Huang R, Lai M, Shih H, Lin S. A robust platform for expansion and genome editing of primary human natural killer cells. J Exp Med. 2021;218: pubmed publisher
Cruz Becerra G, Kadonaga J. Enhancement of homology-directed repair with chromatin donor templates in cells. elife. 2020;9: pubmed publisher
Roth T, Puig Saus C, Yu R, Shifrut E, Carnevale J, Li P, et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature. 2018;559:405-409 pubmed publisher
product information
Catalog Number :
112012
Product Name :
RAB11A-GFP HDRT Source (pTR 143)
article :
doi10.1038/s41586-018-0326-5
id28195828
pubmed_id29995861
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size2686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Synthetic Biology
growth notes :
gRNA sequence that can be used with this DNA template: GGTAGTCGTACTCGTCGTCG
growth strain :
DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYL8
geneRAB11A
id8766
genbank_ids
mutation
nameRAB11A-GFP HDRT
shRNA_sequenceGGTAGTCGTACTCGTCGTCG
size1350
species
9606
Homo sapiens
tags
resistance markers :
3968
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA