This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCRII-TOPO-ZFERV
catalog :
11192
citations: 1
Reference
Shen C, Steiner L. Genome structure and thymic expression of an endogenous retrovirus in zebrafish. J Virol. 2004;78:899-911 pubmed
product information
Catalog Number :
11192
Product Name :
pCRII-TOPO-ZFERV
article :
doi10.1128/JVI.78.2.899-911.2004
id481
pubmed_id14694121
bacterial resistance :
Ampicillin
cloning :
backbonepCRII-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size3950
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish
growth notes :
ZFERV PCR insert (the single 3' A overhangs on both ends are not included): 5'-TCTAAAGGAAAATGAACTTAACAGTTGCGAG (31 nt total upstream of the ZFERV sequence), CGAGgacaac agtggaatgg aatctttgtt aaatttagca aagtacagta aactgaacag agtattgcga ata -3' (73 nt total downstream of the ZFERV sequence). A map of pCRII-TOPO is available at Addgene's vector DB.
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3TOPO TA cloned
cloning_site_5TOPO TA cloned
promoter
sequencing_primer_3T7
sequencing_primer_5Sp6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZFERV, si:dkey-266f7.7, wu:fd52b06, wu:fi23c07, zmp:0000000535
geneerv
id108180146
genbank_ids
AF503912
mutation
nameZFERV
shRNA_sequence
size11249
species
7955
Danio rerio
tags
resistance markers :
500
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA