This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEMS2242
catalog :
111897
citations: 1
Reference
Simpson E, Korecki A, Fornes O, McGill T, Cueva Vargas J, Agostinone J, et al. New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Hum Gene Ther. 2019;30:257-272 pubmed publisher
product information
Catalog Number :
111897
Product Name :
pEMS2242
article :
doi10.1089/hum.2018.118
id18427
pubmed_id30062914
bacterial resistance :
Ampicillin
cloning :
backbonepEMS2131
backbone_mutation
backbone_originElizabeth Simpson (Addgene plasmid # 111895)
backbone_size4999
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth notes :
. Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.
growth strain :
ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterPle321 (TUBB3 MiniPromoter)
sequencing_primer_3WPRE-R
sequencing_primer_5M13-rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
geneTUBB3
id10381
genbank_ids
mutation
namePle321
shRNA_sequence
size1829
species
9606
Homo sapiens
tags
resistance markers :
928
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA