This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDNA2.0 6H-TEV-KRAS G12V
catalog :
111850
citations: 1
Reference
Hunter J, Manandhar A, Carrasco M, Gurbani D, Gondi S, Westover K. Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations. Mol Cancer Res. 2015;13:1325-35 pubmed publisher
product information
Catalog Number :
111850
Product Name :
pDNA2.0 6H-TEV-KRAS G12V
article :
doi10.1158/1541-7786.MCR-15-0203
id28192236
pubmed_id26037647
bacterial resistance :
Kanamycin
cloning :
backbonepDNA2.0
backbone_mutation
backbone_originATUM
backbone_size3939
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
origin :
37
pi :
alt_names
Kirsten ras oncogene G12V
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT5
sequencing_primer_3GAACTGCCAGGCATCAAATAAAA
sequencing_primer_5GGAATTGTGAGCGCTCACAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationChanged Glycine 12 to Valine
nameKRAS G12V
shRNA_sequence
size507
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis-tag
locationN terminal on backbone
tagTEV protease cleavage sequence
resistance markers :
3966
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA