This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDNA2.0 6H-TEV-KRAS
catalog :
111849
citations: 2
Reference
Ratnasinghe B, Haque N, Wagenknecht J, Jensen D, Esparza G, Leverence E, et al. Beyond Structural Bioinformatics for Genomics with Dynamics Characterization of an Expanded KRAS Mutational Landscape. bioRxiv. 2023;: pubmed publisher
Hunter J, Manandhar A, Carrasco M, Gurbani D, Gondi S, Westover K. Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations. Mol Cancer Res. 2015;13:1325-35 pubmed publisher
product information
Catalog Number :
111849
Product Name :
pDNA2.0 6H-TEV-KRAS
article :
doi10.1158/1541-7786.MCR-15-0203
id28192236
pubmed_id26037647
bacterial resistance :
Kanamycin
cloning :
backbonepDNA2.0
backbone_mutationNone
backbone_originATUM
backbone_size3939
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
origin :
37
pi :
alt_names
Kirsten ras oncogene
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT5
sequencing_primer_3GAACTGCCAGGCATCAAATAAAA
sequencing_primer_5GGAATTGTGAGCGCTCACAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutation
nameKRAS
shRNA_sequence
size507
species
9606
Homo sapiens
tags
location
tag6xHis-tag
location
tagTEV protease cleavage sequence
resistance markers :
3966
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA