This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCRISPR_RAF1_94
catalog :
111826
citations: 1
Reference
Sinha H, Quach A, Vo P, Shih S. An automated microfluidic gene-editing platform for deciphering cancer genes. Lab Chip. 2018;18:2300-2312 pubmed publisher
product information
Catalog Number :
111826
Product Name :
pCRISPR_RAF1_94
article :
doi10.1039/c8lc00470f
id28193511
pubmed_id29989627
bacterial resistance :
Ampicillin
cloning :
backboneAll_in_one_CRISPR/Cas9_LacZ
backbone_mutationRemoval of LacZ-alpha in the cloning process
backbone_originLynne Postovit (Addgene plasmid # 74293)
backbone_size10694
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
CRISPR
growth strain :
Knockout Raf1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterU6
sequencing_primer_3
sequencing_primer_5SP6 Forward: ATTTAGGTGACACTATAGA
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
mutation
namegRNA targeting RAF1 94-114
shRNA_sequenceGCCGCCCGAGAGTCTTAATCG
size
species
9606
Homo sapiens
tags
location
tagmCherry
resistance markers :
3612
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA