pcDNA cyclin D1 HA T286A | Addgene 11182 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA cyclin D1 HA T286A

catalog :

11182

citations: 6

Reference
B xe9 langer F, Roussel C, Sawchyn C, St Hilaire E, Gezzar Dandashi S, Kimenyi Ishimwe A, et al. A genome-wide screen reveals that Dyrk1A kinase promotes nucleotide excision repair by preventing aberrant overexpression of cyclin D1 and p21. J Biol Chem. 2023;299:104900 pubmed publisher
Samara A, Shapira S, Lubin I, Shpilberg O, Avigad S, Granot G, et al. Deferasirox induces cyclin D1 degradation and apoptosis in mantle cell lymphoma in a reactive oxygen species- and GSK3β-dependent mechanism. Br J Haematol. 2021;: pubmed publisher
Wang N, Wang X, Tan H, Li S, Tsang C, Tsao S, et al. Berberine Suppresses Cyclin D1 Expression through Proteasomal Degradation in Human Hepatoma Cells. Int J Mol Sci. 2016;17: pubmed
Mohanty A, Sandoval N, Das M, Pillai R, Chen L, Chen R, et al. CCND1 mutations increase protein stability and promote ibrutinib resistance in mantle cell lymphoma. Oncotarget. 2016;7:73558-73572 pubmed publisher
Santra M, Wajapeyee N, Green M. F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage. Nature. 2009;459:722-5 pubmed publisher
Newman R, Mobascher A, Mangold U, Koike C, Diah S, Schmidt M, et al. Antizyme targets cyclin D1 for degradation. A novel mechanism for cell growth repression. J Biol Chem. 2004;279:41504-11 pubmed

product information

Catalog Number :

11182

Product Name :

pcDNA cyclin D1 HA T286A

article :

doi	10.1074/jbc.M407349200
id	459
pubmed_id	15277517

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3?

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Constructed using site-directed mutagenesis on the parent plasmid, pcDNA cyclinD1 HA, using the primers 5'-acctggcttgcgcacccaccgacg-3' and 5'-cgtcggtgggtgcgcaagccaggt-3'. Note: The author's map and author's sequence are not fully correct. EcoRI and XhoI are not unique sites in this plasmid.

origin :

pi :

alt_names

cyclin D1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL1, D11S287E, PRAD1, U21B31
gene	CCND1
id	595

genbank_ids

BC025302

mutation

T286A. Mutant that cannot be degraded via the ubiquitin pathway.

name

cyclin D1 T286A

shRNA_sequence

size

1120

species

9606

Homo sapiens