This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19
catalog :
111741
citations: 1
Reference
Harding R, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, et al. Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. J Biol Chem. 2019;294:6986-7001 pubmed publisher
product information
Catalog Number :
111741
Product Name :
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19
article :
doi10.1074/jbc.RA118.007204
id28193494
pubmed_id30842263
bacterial resistance :
Ampicillin
cloning :
backbonepBacMam2-DiEx-LIC
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Baculovirus Expression
growth notes :
Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
growth strain :
Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
origin :
37
pi :
alt_names
huntingtin
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV and P10
sequencing_primer_3pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')
sequencing_primer_5pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutationQ19 (polyQ repeat)
nameHTT
shRNA_sequence
size9420
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG
plasmid copy :
Kazusa DNA Research Institute Gene KIEE4296 & Original Clone ID cp94296
resistance markers :
732
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA