This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV_puro_41589
catalog :
111632
product information
Catalog Number :
111632
Product Name :
pMSCV_puro_41589
article :
doi
id28193479
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-puro
backbone_mutation
backbone_originClontech
backbone_size6264
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Cloning performed in 2 steps: 1) PCR amplification of the original plasmid using primers featuring an overhang containing an XhoI restriction site, 2) Digestion of the pMSCV puro plamid by XhoI and ligation of the insert
growth strain :
Complementation of Dicer_KO mESCs with mutated human DICER isoform
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_35' attcgttaacctcgagtcagctattgggaa cctgag 3'
sequencing_primer_55' aattagatctctcgagatggactacaa agacgatgacg 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
geneDICER1
id23405
genbank_ids
mutationK70A
nameFLAG-hsDicer (K70A)
shRNA_sequence
size5808
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
plasmid copy :
FLAG-hsDicer (K70A) from addgene plasmid #41589
resistance markers :
2514
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA