This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIH-mRuby2-BAK
catalog :
111627
citations: 1
Reference
McArthur K, Whitehead L, Heddleston J, Li L, Padman B, Oorschot V, et al. BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. Science. 2018;359: pubmed publisher
product information
Catalog Number :
111627
Product Name :
pMIH-mRuby2-BAK
article :
doi10.1126/science.aao6047
id28193477
pubmed_id29472455
bacterial resistance :
Ampicillin
cloning :
backbonepMIH/MSCV-IRES-hygro
backbone_mutation
backbone_originMark Van Delft
backbone_size7005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BglII
promoterMSCV
sequencing_primer_3
sequencing_primer_5gaacctcctcgttcgaccc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBak, N-BAK1, N-Bak
geneBak1
id12018
genbank_ids
NM_007523.3
mutation
nameBAK
shRNA_sequence
size1317
species
10090
Mus musculus
tags
locationN terminal on insert
tagmRuby2
plasmid copy :
Backbone provided by Dr Mark Van Delft & Prof David Huang.
resistance markers :
4238
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA