This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-PARP1-3xFlag-7xKQ
catalog :
111576
citations: 1
Reference
Liszczak G, Diehl K, Dann G, Muir T. Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Nat Chem Biol. 2018;14:837-840 pubmed publisher
product information
Catalog Number :
111576
Product Name :
pCMV-PARP1-3xFlag-7xKQ
article :
doi10.1038/s41589-018-0097-1
id28193460
pubmed_id30013063
bacterial resistance :
Kanamycin
cloning :
backboneCMV
backbone_mutationN-terminal 3xFlag insertion
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells
origin :
37
pi :
alt_names
Poly(ADP-ribose) polymerase 1
ARTD1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
genePARP1
id142
genbank_ids
mutationlysine to glutamine mutation at the following amino acids: 498, 505, 506, 508, 518, 521, 524
namePARP1
shRNA_sequence
size3042
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFlag
resistance markers :
3952
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA