This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-PARP1-3xFlag-WT
catalog :
111575
citations: 9
Reference
Yu C, Sheng Y, Yu F, Ni H, Qiu A, Huang Y, et al. Foxm1 haploinsufficiency drives clonal hematopoiesis and promotes a stress-related transition to hematologic malignancy in mice. J Clin Invest. 2023;133: pubmed publisher
Yang S, Hwang S, Kim B, Shin S, Kim M, Jeong S. Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation. Cell Death Dis. 2023;14:435 pubmed publisher
Concei xe7 xe3 o C, Salgueiro B, Ribeiro P, Raposo M, Moe E. Advances in the expression and purification of human PARP1: A user-friendly protocol. Protein Expr Purif. 2023;211:106336 pubmed publisher
Kim C, Wang X, Jang S, Yu Y. PARP1 inhibitors induce pyroptosis via caspase 3-mediated gasdermin E cleavage. Biochem Biophys Res Commun. 2023;646:78-85 pubmed publisher
Yang F, Chen J, Liu B, Gao G, Sebastian M, Jeter C, et al. SPINDOC binds PARP1 to facilitate PARylation. Nat Commun. 2021;12:6362 pubmed publisher
Sun Y, Chen J, Huang S, Su Y, Wang W, Agama K, et al. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021;12:5010 pubmed publisher
Kim C, Wang X, Yu Y. PARP1 inhibitors trigger innate immunity via PARP1 trapping-induced DNA damage response. elife. 2020;9: pubmed publisher
Pecoraro A, Carotenuto P, Russo G, Russo A. Ribosomal protein uL3 targets E2F1 and Cyclin D1 in cancer cell response to nucleolar stress. Sci Rep. 2019;9:15431 pubmed publisher
Liszczak G, Diehl K, Dann G, Muir T. Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Nat Chem Biol. 2018;14:837-840 pubmed publisher
product information
Catalog Number :
111575
Product Name :
pCMV-PARP1-3xFlag-WT
article :
doi10.1038/s41589-018-0097-1
id28193460
pubmed_id30013063
bacterial resistance :
Kanamycin
cloning :
backboneCMV
backbone_mutationN-terminal 3xFlag insertion
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses full length PARP1 in mammalian cells
origin :
37
pi :
alt_names
Poly(ADP-ribose) polymerase 1
ARTD1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
genePARP1
id142
genbank_ids
mutation
namePARP1
shRNA_sequence
size3042
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFlag
resistance markers :
3952
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA