This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pACEBac1-PARP1-Flag-His-7xKQ
catalog :
111573
citations: 1
Reference
Liszczak G, Diehl K, Dann G, Muir T. Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Nat Chem Biol. 2018;14:837-840 pubmed publisher
product information
Catalog Number :
111573
Product Name :
pACEBac1-PARP1-Flag-His-7xKQ
article :
doi10.1038/s41589-018-0097-1
id28193460
pubmed_id30013063
bacterial resistance :
Gentamicin
cloning :
backbonepACEBac1
backbone_mutationC-terminal Flag-6xHis
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
origin :
37
pi :
alt_names
Poly(ADP-ribose) polymerase 1
ARTD1
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BglII (on insert), BamHI (in vector)
promoterpolyhedrin promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5Custom (gtatcgattcgcgacctactcc)
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
genePARP1
id142
genbank_ids
mutationlysine to glutamine mutation at the following amino acids: 498, 505, 506, 508, 518, 521, 524
namePARP1
shRNA_sequence
size3042
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagC-terminal 6xHis
locationC terminal on backbone
tagC-terminal Flag
resistance markers :
3952
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA