This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRho-DsRed
catalog :
11156
citations: 12
Reference
Herrera I, Fernandes J, Shir Mohammadi K, Levesque J, Mattar P. Lamin A upregulation reorganizes the genome during rod photoreceptor degeneration. Cell Death Dis. 2023;14:701 pubmed publisher
Travis A, Manocha S, Willer J, Wessler T, Skiba N, Pearring J. Disrupting the ciliary gradient of active Arl3 affects rod photoreceptor nuclear migration. elife. 2023;12: pubmed publisher
Sun D, Sun W, Gao S, Lehrer J, Naderi A, Wei C, et al. Effective gene therapy of Stargardt disease with PEG-ECO/pGRK1-ABCA4-S/MAR nanoparticles. Mol Ther Nucleic Acids. 2022;29:823-835 pubmed publisher
Sun D, Schur R, Sears A, Gao S, Vaidya A, Sun W, et al. Non-viral Gene Therapy for Stargardt Disease with ECO/pRHO-ABCA4 Self-Assembled Nanoparticles. Mol Ther. 2020;28:293-303 pubmed publisher
Yao K, Qiu S, Wang Y, Park S, Mohns E, Mehta B, et al. Restoration of vision after de novo genesis of rod photoreceptors in mammalian retinas. Nature. 2018;560:484-488 pubmed publisher
Sarin S, ZUNIGA SANCHEZ E, Kurmangaliyev Y, Cousins H, Patel M, Hernandez J, et al. Role for Wnt Signaling in Retinal Neuropil Development: Analysis via RNA-Seq and In Vivo Somatic CRISPR Mutagenesis. Neuron. 2018;98:109-126.e8 pubmed publisher
Salinas R, Pearring J, Ding J, Spencer W, Hao Y, Arshavsky V. Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release. J Cell Biol. 2017;216:1489-1499 pubmed publisher
Spencer W, Pearring J, Salinas R, Loiselle D, Skiba N, Arshavsky V. Progressive Rod-Cone Degeneration (PRCD) Protein Requires N-Terminal S-Acylation and Rhodopsin Binding for Photoreceptor Outer Segment Localization and Maintaining Intracellular Stability. Biochemistry. 2016;55:5028-37 pubmed publisher
Pearring J, Spencer W, Lieu E, Arshavsky V. Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking. elife. 2015;4: pubmed publisher
Pearring J, Lieu E, Winter J, Baker S, Arshavsky V. R9AP targeting to rod outer segments is independent of rhodopsin and is guided by the SNARE homology domain. Mol Biol Cell. 2014;25:2644-9 pubmed publisher
Gospe S, Baker S, Kessler C, Brucato M, Winter J, Burns M, et al. Membrane attachment is key to protecting transducin GTPase-activating complex from intracellular proteolysis in photoreceptors. J Neurosci. 2011;31:14660-8 pubmed publisher
Matsuda T, Cepko C. Electroporation and RNA interference in the rodent retina in vivo and in vitro. Proc Natl Acad Sci U S A. 2004;101:16-22 pubmed
product information
Catalog Number :
11156
Product Name :
pRho-DsRed
article :
doi10.1073/pnas.2235688100
id474
pubmed_id14603031
bacterial resistance :
Ampicillin
cloning :
backbonepCAGEN
backbone_mutation
backbone_origin
backbone_size3086
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The rhodopsin promoter is specific for photoreceptor cells in the retina.
origin :
37
pi :
alt_names
Bos taurus Rho
Rho promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SpeI
promoter
sequencing_primer_3DsRed-R
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneRHO
id509933
genbank_ids
mutation
namerhodopsin promoter
shRNA_sequence
size2239
species
9913
Bos taurus
tags
locationC terminal on backbone
tagDsRed2
plasmid copy :
The bovine rhodopsin promoter was from -2174-lacZ (Zack et al., Neuron 6, 187-199 (1991)) obtained from Dr. D. Zack (Johns Hopkins University).
resistance markers :
71
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA