pCMV-Tag2-Sox9 T236A | Addgene 111455 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-Tag2-Sox9 T236A

catalog :

111455

citations: 1

Reference
Hong X, Liu W, Song R, Shah J, Feng X, Tsang C, et al. SOX9 is targeted for proteasomal degradation by the E3 ligase FBW7 in response to DNA damage. Nucleic Acids Res. 2016;44:8855-8869 pubmed

product information

Catalog Number :

111455

Product Name :

pCMV-Tag2-Sox9 T236A

article :

doi	10.1093/nar/gkw748
id	23747
pubmed_id	27566146

bacterial resistance :

Kanamycin

cloning :

backbone

pCMV

backbone_mutation

backbone_origin

Stratagene

backbone_size

4300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Reference for original WT SOX9 plasmid - https://www.ncbi.nlm.nih.gov/pubmed/?term=20651055 We used these primers for SDM Sox9 F7site5mut1F: 5'-ggcccaccggccccacccAccAcccccaaaa-3' Sox9 F7site5mut1R: 5'-ttttgggggTggTgggtggggccggtgggcc-3'

growth strain :

Transient expression of Flag-tagged human Sox9 with T236A mutation

origin :

pi :

alt_names

SRY-box 9

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CMD1, CMPD1, SRA1, SRXX2, SRXY10
gene	SOX9
id	6662

genbank_ids

mutation

T236A mutation

name

Sox9

shRNA_sequence

size

species

9606

Homo sapiens