This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-30a(+)-C-SH2+IA
catalog :
111418
citations: 1
Reference
Feng C, Roy A, Post C. Entropic allostery dominates the phosphorylation-dependent regulation of Syk tyrosine kinase release from immunoreceptor tyrosine-based activation motifs. Protein Sci. 2018;27:1780-1796 pubmed publisher
product information
Catalog Number :
111418
Product Name :
pET-30a(+)-C-SH2+IA
article :
doi10.1002/pro.3489
id28193442
pubmed_id30051939
bacterial resistance :
Kanamycin
cloning :
backbonepET-30a(+)
backbone_mutation
backbone_originNovagen
backbone_size5422
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
MFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKL
IATTAHEKMPWFHGNISRDESEQTVLIGSKTNGKFLIRA
RDNSGSYALCLLHEGKVLHYRIDRDKTGKLSIPEGKKFD
TLWQLVEHYSYKPDGLLRVLTVPCQKIGAQ
encoding the following protein sequence:
growth strain :
express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)
origin :
37
pi :
alt_names
Spleen tyrosine kinase (C)SH2 domain plus interdomain A
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho I
cloning_site_5Nde I
promoterT7 promoter
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSykb
geneSyk
id20963
genbank_ids
NP_035648
mutation
namemurine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag
shRNA_sequence
size453
species
10090
Mus musculus
tags
resistance markers :
3948
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA